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KMID : 0613820020120010087
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Journal of Life Science
2002 Volume.12 No. 1 p.87 ~ p.95
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Isolation and Characterization of Complement System Activating Polysaccharides from the Hot Water Extract of the Leaves of Capsicum annuum L.
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Ra Kyung-Soo
Jeong Sang-Chul
Suh Hyung-Joo
Park Hee-Sung
Baik Hyung-Suk
Choi Jang-Won
Lee Yong-Se
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Abstract
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It was observed that the hot water extract of the leaves of Capsicum annuum L., a Korean edible plant, had a potent anti-complementary activity. Crude polysaccharide fraction(CAL-0) was obtained by methanol reflux, ethanol precipitation, dialysis and lyophilization. CAL-0 contained 51.8% of total sugar, 8.2% of uronic acid and 16.8% of protein, and consisted of mainly arabinose, galactose and glucose as neutral sugars and galacturonic acid as uronic acid. The anti- complementary activity of CAL-0 decreased greatly by periodate oxidation, but was not changed by pronase treatment. Also, the anti-complementary activity of CAL-0 was reduced partially in the absence of the Ca2+ ion. The crude polysaccharide CAL-0 was found to activate the C3 component both in the presence and in the absence of Ca2+ through the crossed- immunoelectrophoresis suggesting that those involved in both classical and alternative complement pathway. CAL-0 was further separated to an unabsorbed fraction(CAL-1) and six absorbed fractions(CAL-2→CAL-7) on DEAE Sepharose CL-6B ion exchange column. Among them four major fractions in activity and yield were obtained, and consisted mainly of arabinose, galactose and glucose with various molar ratios. The major fraction, CAL-2, was purified to give a high molecular fraction(CAL-2-I) and a low molecular fraction(CAL-2-II) on Sepharose CL-6B column. The anti- complementary activity of CAL-2-I, a molecular weight of about 61,000, was higher than it of CAL-2-II.
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KEYWORD
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leaves of Capsicum annuum L., anti-complementary activity, polysaccharide
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